This assay provides information about red cells, white cells and platelets including new parameters of platelets like Fluorescent platelets (Platelet-F), Immature platelet fraction (IPF), Mean platelet volume (MPV), Platelet distribution width (PDW) which provide complete indepth information about platelets and also includes automated Immature Granulocyte Count (IG) in differential count of WBC, providing more accurate count of immature granulocytes as compared to manual count. Results are useful in the diagnosis of anemia, infections, leukemias, clotting disorders and many other medical conditions.