Dr. Nikunj Jain
Co-Founder and HOD - Nuclear Medicine ,MBBS, DRM, DNB, FEBNM, FANMB, Dip. CBNC
Discovering whether you’re pregnant can be one of life’s most emotional moments — exciting for some, nerve-wracking for others. Thanks to modern diagnostics, pregnancy tests today are simple, fast, and highly accurate.
But do you know how pregnancy tests actually work, when you should take them, or what might cause a false result?
Let’s explore the science and truth behind pregnancy tests in a simple, conversational way.
A pregnancy test detects a hormone called human chorionic gonadotropin (hCG) in your body.
This hormone is produced by the placenta shortly after a fertilized egg attaches to the uterine lining — usually about 6–10 days after conception.
When hCG is detected in your urine or blood, it’s a strong sign that pregnancy has begun.
Pregnancy tests are designed to detect the presence of hCG — often called the pregnancy hormone.
When fertilization occurs, hCG levels rise rapidly, doubling every 48–72 hours in the early weeks.
Tests use antibodies that bind specifically to hCG. When the hormone is present, a visible line or signal appears on the test — confirming pregnancy.
There are two main types of pregnancy tests: urine tests and blood tests.
Let’s understand the difference.
The most common type — and the one you can do privately at home.
Done in diagnostic laboratories or clinics, this test measures exact hCG levels in your blood.
There are two types:
Timing matters!
The best time to take a pregnancy test is after you miss your period.
Taking the test too early may result in a false negative, as hCG levels might not be high enough to detect yet.
Sometimes, pregnancy tests can show false positives or false negatives. Here’s why:
Most home pregnancy kits claim to be 99% accurate when used correctly after a missed period.
However, real-world accuracy depends on:
Blood tests are the most reliable method — nearly 100% accurate — since they measure exact hCG levels.
If your test is positive:
A negative test doesn’t always mean you’re not pregnant.
If your period still doesn’t start within a few days:
Taking a pregnancy test can bring many emotions — hope, anxiety, or confusion.
No matter the outcome, remember: you’re not alone.
Speak to a healthcare professional for guidance, support, and next steps.
You can take a blood test about 10 days after ovulation or a urine test after a missed period.
Yes. When used correctly after a missed period, they are up to 99% accurate.
Yes, if taken too early or incorrectly. Always follow instructions and confirm with a lab test.
Early morning urine gives the most accurate results since it’s more concentrated.
Even a faint line often indicates pregnancy — test again in 2–3 days to confirm.
Yes. Fertility drugs containing hCG can cause false positives.
Immediately — early prenatal care is crucial for a healthy pregnancy.
Yes. Stress can delay your cycle but doesn’t affect hCG production once pregnant.
Yes, but morning testing is more accurate.
Consult your doctor and get a blood test for confirmation.
A pregnancy test is more than just a simple diagnostic tool — it’s the first step in your motherhood journey or fertility awareness.
Whether taken at home or in a lab, accurate testing and medical follow-up ensure your safety and peace of mind.
At Molecular Diagnostics, we provide precise, lab-based pregnancy tests and other fertility diagnostics to support your reproductive health every step of the way.
Book tests, view reports, and manage your health records on the go. Experience convenient healthcare with Molecular Diagnostics and Therapy.